New to HPLC? Tips for Avoiding Beginner Pitfalls

Operating an HPLC (High-Performance Liquid Chromatography) system can be overwhelming for a new user. There are numerous pitfalls or “gotchas.” Even the matter of which tubing and fittings are compatible, and which are not, can pose a serious impediment to successfully operating the HPLC.

In this discussion, we will cover:

  • The significance of connections
  • Considerations for the mobile phase
  • Quality considerations
  • Preventing microbial growth
  • Compatibility of sample and solvent
  • Characteristics of the column; their significance and limitations
  • The importance of sample preparation

 

Speakers

Rita Steed
Application Engineer
Agilent Technologies, Inc.

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.

Register and watch on demand >

 

Food Testing Workflows Webinar Series

Ensuring food safety and quality is more crucial than ever. For African countries, where ensuring food safety and quality is paramount for the well being of their citizens and the success of their exports, Agilent expertise can make a difference.

Chemetrix offers Agilent’s comprehensive suite of solutions that helps you identify pesticide residues, verify label claims, and safeguard food authenticity. Now, a new free-to-access series of webinars expands on necessary knowledge for this valuable process that ensures our food is safe and nutritious.

These webinars are designed to help you learn more about the latest trends and technologies in Food Testing Workflows. Agilent experts will present webinars on the latest applications, soil analysis, laboratory performance improvement, time and cost savings opportunities, and much more.

 

 

Webinar 1 – Agilent LC Solutions for Food Testing and Food Fortification

HPLC is a powerful analytical technique widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis

Speaker

Ansuman Mahato
Application Engineer – HPLC
Agilent Technologies

 

 

Webinar 2 – Latest Applications and Workflows for the Food Market

Food laboratories find themselves in a frequently changing environment where they need to update their analytical procedures to follow new legislation and upcoming regulations.

Speaker

Cecilia Mazza
Product Specialist, EMEA IDO – Chemistries & Suppliers
Agilent Technologies

 

 

Webinar 3 – Sample Prep for Food Analysis: An overview about available techniques

In this presentation we will cover most common Sample Preparation Techniques for Food Analysis and related products, as well as most advanced, specific and unique solutions.

Speaker

Giorgio Ferlat
MSc, EMEAI IDO Product Specialist, Chemistries and Supplies
Agilent Technologies

 

 

Webinar 4 – Multiresidue Pesticide Analysis in Food using GC/MS/MS and LC/MS/MS in Accordance with the SANTE 11312/2021 Guideline

Pesticides play an important role in the agriculture and food industries to improve crop yield and food production. However, the quantity of pesticide residue remaining in or on commodities are tightly controlled to avoid contamination of the food chain and the environment. Therefore, regulatory agencies have set maximum residue levels (MRLs) for hundreds of pesticides and their metabolites.

Speaker

Laurent Pascaud
Spécialiste Produits GC & GC/MS
Agilent Technologies

 

 

Webinar 5 – Plant and Soil Analysis for the Determination of their Inorganic Content

Testing soils and plants for trace and major elements is important for the development, the growth and the plant metabolism. This is also critical for monitoring the plant nutrients composition and assessing the potential for contamination of food crops.

Speaker

Uwe Noetzel
EMEAI Technical Coverage and Solutions
Agilent Technologies

 

 

Webinar 6 – Analysing Trace and Major Elements in Food Samples

To ensure food safety and safeguard human health, the characterization of the elemental composition of a wide range of food types is required. Since the concentration ranges for different elements vary significantly across different foods, various methodes can be used for sample characterization.

Speaker

Uwe Noetzel
EMEAI Technical Coverage and Solutions
Agilent Technologies

 

 

Webinar 7 – Better Lab Sample Turnaround – Saving Time and Solvent per run

Nowadays, laboratories have the challenge of becoming progressively more productive as well as focusing on continuous improvements.

Speaker

Giorgio Ferlat
MSc, EMEAI IDO Product Specialist, Chemistries and Supplies
Agilent Technologies

 

 

Webinar 8 – Quantitative Analysis of Mycotoxins in Foods by Triple Quadrupole LC/MS

Pesticides play an important role in the agriculture and food industries to improve crop yield and food production. However, the quantity of pesticide residue remaining in or on commodities are tightly controlled to avoid contamination of the food chain and the environment. Therefore, regulatory agencies have set maximum residue levels (MRLs) for hundreds of pesticides and their metabolites.

Speaker

Henry Russell
LCMS Product Specialist
Agilent Technologies

 

Agilent food and beverage testing products and services keep you at the forefront of the latest trends, ahead of issues, and focused where you need to be to deliver the highest quality and value to your customers.

Join this informative and exciting webinar series that will explore topics such as the latest applications and workflows for the food market, sample prep for food analysis, plant and soil analysis for inorganic content and much much more. The webinar series is free but registration is required.

 

Register now >

 

OpenLab CDS Webinar Series

Discover what OpenLab CDS software suite can do in your lab!

Seminar 1 – The Control Panel and Data Acquisition

The Control Panel is the gatekeeper to the system. Learn how to enable authentication to allow chemist names to be placed on reports. See how to configure a system in a compliant environment and restrict users’ access to instruments and data. Understand the use of Roles and Privileges to refine what users can do. Understand the need, creation, and use of Projects. Explore how to set up an Acquisition Method for a virtual 3-D HPLC and a virtual 7890 GC. Explore the software features that have been designed to help minimize sequence creation time and eliminate costly typographical errors.

 

Seminar 2 – Data Analysis

Explore the new look and feel of the Data Analysis engine. Become acquainted with Integration Parameters and how to create a calibration curve for quantitation. Investigate new software tools such as Peak Explorer, UV and MS Spectral capabilities, library searching and matching, peak purity, and more. Learn how to combine information from multiple results sets to track trends across time and instrumentation.

 

Seminar 3 – Custom Calculator and Reporting

Stop doing your calculations in Excel. Learn how to set up calculations in the Custom Calculator and Intelligent Reporter. Explore the differences between the two portions of software and understand where and when to use each. Customize individual injection reports. Explore how to create a sequence report with separate sections for standards and samples so statistics like average and standard deviation can be calculated across injections in a sequence.

 

Session 4 – OpenLab CDS for MS Data

OpenLab CDS can control both LC/MS (SQ) and GC/MS (SQ) instruments in your lab. Learn the Data Analysis workflow tips on how to most effectively and efficiently analyze MS-specific data.

 

Speakers

Richard Mutkoski
Laboratory Informatics Application Engineer
Agilent Technologies, Inc.

Richard Mutkoski has been a field representative for over 27 years for both hardware and software. In his current role as an informatics application engineer, Rich presents technical solutions of the OpenLab suite of products to customers worldwide. Prior to his tenure at Agilent Rich spent 15 years in a variety of pharma QA/QC and R&D positions, providing him a unique understanding of those workflows, bottlenecks, and challenges.

Register and watch on demand >

 

Modernise Your Lab with Intelligent Software

To move into the digital age, you need software that can help you manage the growing amount of data generated by the modern lab. The right tools help turn that data into actionable insights and keep your lab operating efficiently. Today’s laboratory software solutions need to easily migrate data and methods from aging platforms and simplify data analysis and reporting tasks.

This webinar explores key features laboratory software must have to meet the demands of today’s laboratory. It also discusses the best process to migrate methods and data to the latest software platform. Another touchpoint is how to increase productivity and accessibility of instruments through a modern cloud-based architecture.

 

Speakers

Kathleen O’Dea
Application Engineer
Agilent Technologies, Inc.

Kathleen O’Dea has a 20-year history with Agilent’s OpenLab products. As an Informatics Application Engineer, Kathleen has visited customers around the world in a wide variety of market areas, helping them select and implement OpenLab software products.

Register and watch on demand >

 

Decoding Automation of Metabolite and Lipid Extraction Workflows

Technology improvements in liquid chromatography/mass spectrometry have enhanced the detection and identification of metabolites and lipids from complex biological samples. As metabolomics and lipidomics measurements become increasingly valued, there is a growing need to automate sample preparation workflows.

Specifically, Agilent automation offers intuitive workflows that provide high data reproducibility and increased throughput while reducing hands-on time. In this webinar, we describe key learnings revealed during the automation of several workflows that extract metabolites and/or lipids from plasma and mammalian cell samples.

 

Speakers

Genevieve Van de Bittner, Ph.D.
R&D Researcher
Agilent Research Laboratories
Agilent Technologies, Inc.

 

Register and watch on demand >

 

Jet Fuel by ICP-MS

The measurement of trace metals in petroleum feeds and its derivatives provides vital information required for running sustainable and daily petroleum operations around the world. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is used in different petroleum facilities due to its ability to perform multi-element analyses, covering a broad range of concentrations as well as being robust and reliable. ICP-MS is becoming more integrated into petroleum laboratories due to its maturity and versatility.

This talk will cover Agilent’s efforts towards developing an ASTM Jet Fuel method. Many interesting elements that aren’t commonly requested, including Platinum (Pt) and Palladium (Pd), will be discussed with this new ICP-MS method. Preliminary data from the ASTM pilot study will be shared in this talk.

 

Speakers

Jenny Nelson, PhD
Application Scientist
Agilent Technologies, Inc.

 

Mark Kelinske
Application Scientist
Agilent Technologies, Inc.

 

Register and watch on demand >

 

Rapid Accurate Detection of Mitochondrial Toxicity

In cell analysis, real-time cell measurements provide a clear window into the critical functions driving cell signalling, proliferation, activation, toxicity and biosynthesis.

In this webinar, Dr. George Rogers discusses Agilent Seahorse technology and workflow advancements that enable simplified assessment of mitochondrial toxicity with high sensitivity and specificity. Points of discussion include the Mito Tox Index (MTI), a standardised parameter reflecting both the magnitude and mode of mitochondrial toxicity, as well as the applications of the Agilent Seahorse XF Mito Tox Assay solution, including examples with HepG2 cells and primary hepatocytes.

 

Speaker

George W. Rogers, Ph.D.
Research Scientist, Expert Bioassay Solutions, Cell Analysis Division
Agilent Technologies, Inc.

 

Register and watch on demand >

 

Unlock your lab’s full potential with CrossLab Asset Monitoring

Managing the business of a lab is a whole other science and it’s time-consuming. Controlling costs, and budget and making sure the laboratory meets its business growth goals is a challenge.

The operational data you collect from your instruments is a valuable tool for improving many areas of performance. However, manually collecting sets of data and relying on paper-based aggregation will not give you a complete understanding of your lab efficiency and productivity.

Agilent CrossLab Asset Monitoring capabilities pull together advanced Internet of Things (IoT) sensor technology and data analytics to enable lab-wide visibility. In this webinar, discover how to recognise the hidden costs of not collecting instrument usage data. It will also explore how to use lab-wide instrument data to make capital expense decisions, reduce operational expenses, and improve productivity. An important point of discussion will be how internet-of-things (IoT) technology is available today to help lab managers measure instrument usage. This webinar is ideal for Lab Operations Managers, Operations Directors or persons in Head of Operations roles.

 

Speakers

Tim Baxter
Marketing Manager
CrossLab Connect Asset Monitoring
Agilent Technologies

 

Michael Farrell
Project Manager, Data Visualization and Insights
Agilent Technologies

 

Moderator

Laura Bush
Editorial Director
LCGC

 

Register and watch on demand >

 

Avoiding Common Time Traps in ICP-MS Analysis: A Virtual Workshop

Inductively coupled plasma–mass spectrometry (ICP-MS) is a fast, multielement technique used for trace elemental analysis.

But labs that use ICP-MS – or are thinking of installing one – can find it difficult to unlock the true potential of the technique. Unproductive and often unnecessary activities can eat into lab time, reducing productivity, increasing stress, and potentially impacting data quality. Open to all; this workshop will provide insights you can employ to improve efficiency in your laboratory while also reducing pressure on staff and increasing confidence in the results you report.

 

Speakers

Bert Woods
Application Scientist
Agilent Technologies, Inc.

Joined the Agilent ICP-MS team in 2004, with previous employment in the semiconductor industry with Dominion Semiconductor (IBM/Toshiba) and Micron. Bert is a 1997 Chemistry graduate of Radford University in Virginia and an avid Washington DC Sports fan.

 

L. Craig Jones
ICP-MS Application Scientist
Agilent Technologies, Inc.

Craig has been with Agilent for over 15 years as an ICP-MS applications scientist. He has been involved with multiple types of applications for ICP-MS, including environmental, pharmaceutical, nutraceutical, semiconductor, geologic, and clinical analyses, to name a few. Previous to Agilent, he worked in an environmental lab performing analysis and supervising both the inorganic and organic sections of the laboratory. In his spare time, Craig enjoys volunteering at the local marine science centre, mountain biking, hiking and relaxing at the beach. Craig obtained a bachelor of science degree in chemistry from Fort Lewis College in Durango, CO.

 

Register and watch on demand >

 

The Efficiency and Cost Benefits of an Innovative UV-Vis Spectrophotometer

UV-Vis spectroscopy is a mature technology used to analyse, characterise, and quantify pharmaceutical and biological samples such as active pharmaceutical ingredients, DNA/RNA, and proteins for many decades. The use of UV-Vis has been limited by the workflow needed to make these measurements efficiently. The recent advances in UV-Vis spectroscopy focus on enhancing laboratory productivity, offering ease of use, and providing multiple accessories designed specifically for application needs.

Pharmaceutical and biopharmaceutical materials have become more sophisticated in life science research across fields (such as cancer research, drug development, vaccines, and quality control in regulated environments). The technology used for the analysis should evolve, too.

This webinar will highlight the benefit of the new Agilent Cary 3500 Flexible UV-Vis spectrophotometer and its capabilities in improving workflows in the pharmaceutical industry.

 

Speaker

Geethika Weragoda
Application Scientist
Agilent Technologies, Australia

 

Register and watch on demand >