Tag: Liquid Chromatography

The water we have on Earth is finite. Although we have water in abundance, caring for this resource has been one of the world’s most pressing environmental challenges. Sadly, we simply do not know the vast majority of chemicals that are discharged into the environment through human activities. For this reason, the detection and identification of these compounds are essential for accurate toxicological profiling of environmental samples.

Ensuring water quality and safety through analytical testing is crucial for public health and environmental protection. Comprehensive testing involves analysing regulated pollutants, including pesticides, semi-volatile organic compounds, metals, and disinfection byproducts. It also extends to emerging contaminants such as PFAS, microplastics, hormones, and various unknown chemicals.

As environmental challenges continue to evolve, detecting and identifying emerging contaminants in water has become a critical task for researchers. Advanced analytical technologies, such as high-resolution mass spectrometry (HRMS), gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS), play a pivotal role in this effort. These sophisticated instruments not only enhance the detection capabilities but also contribute to a deeper understanding of the toxicological impacts of unknown chemicals.

The role of advanced analytical technologies

High-Resolution Mass Spectrometry (HRMS)

HRMS provides unparalleled precision and accuracy in measuring the mass of chemical compounds. It allows for the detection of a wide range of contaminants, even those present at trace levels. This technology is particularly beneficial for non-targeted analysis, where the goal is to identify unknown compounds in water samples. By delivering high-resolution data, HRMS enables researchers to pinpoint the exact mass of contaminants, facilitating their identification and characterisation.

Watch our webinar on Using Liquid Chromatography with QTOF High-Resolution Mass Spectrometry to Identify Emerging Contaminants in Urban Waters >

Gas Chromatography-Mass Spectrometry (GC-MS)

GC-MS is a powerful tool for separating and analysing volatile and semi-volatile organic compounds. It combines the separation capabilities of gas chromatography with the detection prowess of mass spectrometry. This technology is essential for identifying contaminants that may not be detectable through other means. GC-MS excels in providing detailed information about the chemical composition of water samples, making it indispensable for comprehensive water quality assessments.

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

LC-MS/MS is renowned for its sensitivity and specificity in detecting and quantifying contaminants. This technology is particularly effective for analysing non-volatile and polar compounds that are challenging to detect with GC-MS. LC-MS/MS allows researchers to conduct multi-residue analysis, detecting multiple contaminants simultaneously. Its high throughput and precision make it a cornerstone in environmental monitoring and toxicological studies.

New threats emerging

Microplastics are tiny synthetic particles or polymeric matrices derived from plastic, ranging from 1 µm to 5 mm in size and insoluble in water. According to an article published by Agilent, current research believes that microplastics will also degrade into smaller particles on a nanoscale, called ‘nanoplastics’. Despite increasing analysis, their environmental risk remains unclear. The World Health Organisation (WHO) has called for more scientific research to better understand the potential toxicity of microplastics.

Download the infographic poster on Accurate Microplastics Analysis >

A recently published study* stated, “The prevalence of micro and nanoplastics (MNPs) in various environmental and human compartments has highlighted the need for analytical methods to accurately detect and quantify these contaminants. Pyrolysis-gas chromatography coupled with mass spectrometry (Py-GC-MS), one of the thermo-analytical methods, is evolving as an analytical technique to quantify MNPs in complex matrices.”

This study evaluated the impact of using diverse polystyrene (PS) standards with different molecular weights, polydispersity indexes, tacticity, end-capping, and chain branching, on quantifying the mass concentration of PS in various products. The results for the PS-based products showed inconsistencies across different standards, indicating that the measurements for a single product varied substantially when different polystyrene (PS) standards were applied.

The team behind the study made use of Agilent technologies for their research and found there is a need for refined calibration strategies and standardised reference materials to improve the reliability of the MNP analysis method.

From this example, it’s clear that advanced analytical technologies are not only about detection but also about understanding the broader implications of contaminants, like microplastics. By accurately identifying and understanding newer chemicals and contaminants, researchers can assess their toxicological impacts on human health and the environment. This knowledge is crucial for developing effective mitigation strategies and regulatory policies.

Watch our webinar on Microplastics Analysis Just Got Easier: Analysis Direct On-Filter >

Continuous improvement of water analysis

Chemetrix is at the forefront of providing state-of-the-art analytical instruments that empower researchers in their quest to safeguard water quality. By offering cutting-edge technologies such as HRMS, GC-MS, and LC-MS/MS, Chemetrix supports comprehensive environmental research. The instruments are designed to meet the rigorous demands of modern laboratories, ensuring reliable and accurate results.

A prime example of the application of these technologies is non-targeted analysis in water. This approach involves screening water samples for a wide array of contaminants without prior knowledge of their presence. By employing HRMS, GC-MS, and LC-MS/MS, researchers can detect and identify unknown compounds, providing a holistic view of water quality. This method is essential for uncovering emerging contaminants that may not be included in routine monitoring programs.

To preserve our planet’s resources for future generations, the scientific community has to be the trailblazers of today that’ll help find the solutions to protect our tomorrow. There is an incredible amount of passion and dedication among the researchers and scientists who are fighting the good fight against emerging water contaminants and providing valuable insights that everyone can use to make better choices. They can’t do this work without great analytical instruments.

These instruments enhance detection capabilities, provide valuable insights into toxicological impacts, and support informed decision-making. Chemetrix’s commitment to providing cutting-edge solutions underscores its vital role in environmental research. As we continue to face new environmental challenges, the adoption of these advanced technologies will be crucial in ensuring the safety and sustainability of our water resources.

*Quantitation of polystyrene by pyrolysis-GC-MS: The impact of polymer standards on micro and nano plastic analysis by M. Brits, B. van Poelgeest, W. Nijenhuis, M.J.M. van Velzen, F.M. B´een, G.J.M. Gruter, S.H. Brandsma, M.H. Lamoree