New to HPLC? Tips for Avoiding Beginner Pitfalls

Operating an HPLC (High-Performance Liquid Chromatography) system can be overwhelming for a new user. There are numerous pitfalls or “gotchas.” Even the matter of which tubing and fittings are compatible, and which are not, can pose a serious impediment to successfully operating the HPLC.

In this discussion, we will cover:

  • The significance of connections
  • Considerations for the mobile phase
  • Quality considerations
  • Preventing microbial growth
  • Compatibility of sample and solvent
  • Characteristics of the column; their significance and limitations
  • The importance of sample preparation



Rita Steed
Application Engineer
Agilent Technologies, Inc.

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.

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Decoding Automation of Metabolite and Lipid Extraction Workflows

Technology improvements in liquid chromatography/mass spectrometry have enhanced the detection and identification of metabolites and lipids from complex biological samples. As metabolomics and lipidomics measurements become increasingly valued, there is a growing need to automate sample preparation workflows.

Specifically, Agilent automation offers intuitive workflows that provide high data reproducibility and increased throughput while reducing hands-on time. In this webinar, we describe key learnings revealed during the automation of several workflows that extract metabolites and/or lipids from plasma and mammalian cell samples.



Genevieve Van de Bittner, Ph.D.
R&D Researcher
Agilent Research Laboratories
Agilent Technologies, Inc.


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Benefits of Dedicated Bio LC Instruments and Columns

Got 20 minutes to increase your knowledge?


What will you learn

  • Watch this webinar to learn how dedicated UHPLC instrumentation and chromatography columns can help overcome the hurdles of compromised sample integrity and surface interaction.


Preventive and Routine Maintenance for Your HPLC System

Similar to our cars needing regular oil changes, tire pressure adjustments, or washer fluid top ups, our HPLC systems also need routine maintenance in order to keep them performing at their best.  Even if you pay a mechanic to change your oil or a Field Service Engineer to change your pump seals, you should still know how frequently these preventive maintenance activities need to occur.


This presentation will look at several key issues, such as:

  • How often typical preventive maintenance (PM) tasks should be performed.
  • What signs to look for that may indicate a need for maintenance.
  • Tools for testing the LC systems before and after maintenance.
  • Basic troubleshooting and diagnostics.

The goal of this talk is to lay a strong and broad foundation from which to build a better understanding of your own HPLC systems and their specific maintenance needs.


Paul Altiero
Application Engineer
Agilent Technologies, Inc.


Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.


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HPLC Column Care

Are you just getting started with a new column you have never used before? Are you looking for ways to help your columns last a bit longer? These are some of the most common questions asked of our HPLC columns technical support team. In this webinar, we will discuss best practices for HPLC column care, including reversed phase, SEC, HILIC and more. This will include initial equilibration, benchmarking a new column, column storage, and other tips to help make your columns last longer.


Mark Powell
Application Engineer
Agilent Technologies, Inc.


Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.


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Reliability of Multi-residue Identification

It is of utmost importance to ensure that the analytical results from residue testing for food products are reliable. “False positive” findings of hazardous residues in food result in safe product being rejected and leading to potential huge financial and juristic implications. “False negative” findings result in contaminated product being on the shelf and increase the risk of a foodborne disease. The reliability of testing results depends on the type of methodology and the performance criteria. Identification is the important step before a residue is reported. Procedures for identification should be rigorous and depend on sensitivity and selectivity of the MS techniques. The causes of “false positive” and “false negative” derived from both QqQ-MS and HR-MS detection will be discussed. The approaches of establishing a “fit-for-purpose” methodology and identification criteria for both QqQ-MS and HR-MS will be introduced. This will help the testing labs build the protocols/procedures to follow to reduce the incidence of “false detections”; help set up the criteria for identification; and help deliver the highly accurate results.


Hui Zhao
Pre-Sales Application Engineer
Agilent Technologies, Inc.


Hui has 13 years of industry experience developing and validating analytical methods for food/feed nutrition, food safety, dietary supplements and botanicals testing, using a variety of analytical techniques including LC-TQ, LC-QTOF, LC-DAD and GC-MS and a breadth of sample preparation methodologies. She has worked as a Research Scientist at Monsanto, EPL-Bioanalytical Services, Tate & Lyle, Inc. and Lead Staff Scientist at Covance Food Solutions. She holds a Master of Science degree in Analytical Chemistry from Lanzhou University in China and a Ph.D. in Analytical Chemistry from the University of Missouri.

Prior to becoming a Pre-Sales AE, Hui worked as an LCMS application scientist in Agilent’s global market development group where her primary focus was food and environmental market development.


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Liquid Chromatography

Chemetrix InfinityLab solutions for high- and ultrahigh-performance liquid chromatography include instruments, columns, and supplies to help your lab achieve highest operational efficiency. From routine analysis to cutting-edge research, the InfinityLab LC Series offers the broadest portfolio of HPLC, UHPLC and Preparative HPLC solutions for any application and budget.



Learn about liquid chromatography systems and their main components, as well as the basics of LC separation and the common industries where they are used.


HPLC Fundamentals

Learn about liquid chromatography systems and their main components, as well as the basics of LC separation and the common industries where they are used.

High Performance Liquid Chromatography Fundamentals – Theory


Enabling datascience through creation of a chromatography results database

Chromatography Database

The LC Handbook

A Comprehensive Guide to LC Columns and Method Development

LC Handbook


Tips & Tricks

Get tips and tricks for keeping your chromatography alive with an insightful presentation that’ll show you how to bring your column back to life, when it is time to get a new and preventative measures you can take.