Category: Upcoming and New Events

HPLC Column Care

Are you just getting started with a new column you have never used before? Are you looking for ways to help your columns last a bit longer? These are some of the most common questions asked of our HPLC columns technical support team. In this webinar, we will discuss best practices for HPLC column care, including reversed phase, SEC, HILIC and more. This will include initial equilibration, benchmarking a new column, column storage, and other tips to help make your columns last longer.

 

Speaker

Mark Powell
Application Engineer
Agilent Technologies, Inc.

 

Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.

 

Register and watch on demand >

 

Analysis of Nitrosamine Impurities in Pharmaceuticals

What you will learn

  • Understand what mutagenic impurities are and why it’s important to characterize and quantify them, even when present in trace amounts.
  • Understand the current regulatory guidelines for nitrosamines analysis in drug substance and product in Angiotensin II Receptor Blockers (sartan), ranitidine and metformin drugs
  • Learn about approaches for the prevention of the formation of GTIs and the confident identification and quantification of nitrosamines in APIs and drugs

 

Who should attend

  • Laboratory managers
  • Chromatographers
  • Analytical chemists and scientists
  • New product developers
  • Pharma manufacturing and quality control managers

 

Speaker

Raman V.V.S.S. Nanduri, Ph.D.
Director,
Analys Lab Pvt. Lt

 

Register and watch on demand >

 

Sweetening your Sugar Analysis with Electrichemical Detection

In this talk we compare three commonly used techniques for the analysis of sugars. We will show the integration of the Metrohm Electrochemical detector into the Agilent OL CDS software. We will demonstrate the LOD and LOQs of each detector for a series of standards and samples.

 

Speaker

Sue D’Antonio
Applications Scientist
Agilent Technologies, Inc.

 

Sue D’Antonio joined Agilent (HP at that time) more than 30 years ago. For the last 15 years Sue has been an Applications Scientist in the Liquid Phase Separations Group. Sue’s application support extends to HPLC, UHPLC, CE and LCMS across a wide range of markets and customers.

 

Register and watch on demand >

 

Characterisation of Non-Ionic Surfactants

In this talk, we will show characterization of ethoxylation in non – ionic surfactants. In the first dimension the separation is by degree of ethoxylation, while in the second dimension by the separation occurs by the length of the hydrophobic tail. This makes identification and quantitation possible in a single run. Using Agilent 2D LC MassHunter 11 software coupled with the G6230A Accurate Mass Time of flight instrument. We have both quantitative and qualitative data. We can show ppb levels of quantitative detection with Personal Compound Database Library (PCDL) matching in a Walkup environment that a plant QC technician can use.

 

Speaker

Sue D’Antonio
Applications Scientist
Agilent Technologies, Inc.

 

Sue D’Antonio joined Agilent (HP at that time) more than 30 years ago. For the last 15 years Sue has been an Applications Scientist in the Liquid Phase Separations Group. Sue’s application support extends to HPLC, UHPLC, CE and LCMS across a wide range of markets and customers.

 

Register and watch on demand >

 

Per and Polyfluoroalkyl Substances in Soils

Graphitized carbon black (GCB) has been used widely in sample preparation for efficient removal of pigments and other matrix interferences. However, GCB may cause the loss of some analytes. Carbon S is an advanced hybrid carbon material with optimized carbon content and pore structure. Compared to GCB, Carbon S provides equivalent or better pigment removal from sample matrices, while significantly improving recovery for some GCB-selective analytes (such as planar pesticides). As a result, Carbon S sorbent provides a better balance between analyte recovery and matrix removal efficiency than traditional GCB sorbent. The Carbon S sorbent is applied in the same SPE cartridge format with the same bed mass as GCB SPE. The Carbon S SPE cartridges can be used as a replacement for the GCB cartridges for applications where SPE methodology is used. This study investigates the post-extraction matrix cleanup of 59 PFAS from loamy sand, reed sedge peat, and topsoil using the Bond Elut Carbon S 250 mg, 6 mL cartridges followed by LC/MS/MS analysis.

 

Speaker

Matt Giardina
Applications Chemist
Agilent Technologies, Inc.

 

Matthew Giardina is an applications chemist at Agilent Technologies focusing on the development of sample preparation techniques for challenging environmental applications. He has been with Agilent for 10 years and working in the field of analytical chemistry for over 20 years. He received and B.S. in chemistry from the University of Maryland at College Park and Ph.D. in analytical chemistry from the Ohio State University.

 

Register and watch on demand >

 

Customer Care, Quick and Easy

Chemetrix is proud to bring you technology that makes your lab processes more efficient and accurate. Now, we’re doing the same for our customer care with Freshdesk – our new, user-friendly system for customer support.

Freshdesk is customer service software that is easy to use and navigate. All Chemetrix customers can register on Freshdesk for FREE! The software ensures you can easily communicate your questions or technical problems and monitor the help your receive from start to finish.

Step 1:
Create an account

Here’s how it works: Simply log in to Freshdesk or send an email to [email protected].

Step 2:
Logging a ticket

This will create a ticket on the system where you can include any pertinent information from order numbers, relevant documents and to photos as well as the details of your query.

Step 3:
Ticket updates

Your ticket is assigned to Chemetrix personnel with specialist knowledge of your business and the type of instrumentation used in your lab. You are able to see all the communication between yourself and the Chemetrix team, communication between Chemetrix staff as they work to resolve your query as well as to monitor the status of your ticket at all times.

Step 4:
Rate the service

Once your query has been resolved, Chemetrix will resolve the ticket and you’ll receive an email with a link to rate the service.

Benefits

 

    Freshdesk is easy to use and free of charge

    All communication is transparent

   You can monitor the status of your query in real time

   You have a dedicated Chemetrix staff member

Let’s get started

Register on Freshdesk now and discover how Chemetrix can make customer care an efficient, effective and enjoyable experience.

Submit a ticket

 

Reliability of Multi-residue Identification

It is of utmost importance to ensure that the analytical results from residue testing for food products are reliable. “False positive” findings of hazardous residues in food result in safe product being rejected and leading to potential huge financial and juristic implications. “False negative” findings result in contaminated product being on the shelf and increase the risk of a foodborne disease. The reliability of testing results depends on the type of methodology and the performance criteria. Identification is the important step before a residue is reported. Procedures for identification should be rigorous and depend on sensitivity and selectivity of the MS techniques. The causes of “false positive” and “false negative” derived from both QqQ-MS and HR-MS detection will be discussed. The approaches of establishing a “fit-for-purpose” methodology and identification criteria for both QqQ-MS and HR-MS will be introduced. This will help the testing labs build the protocols/procedures to follow to reduce the incidence of “false detections”; help set up the criteria for identification; and help deliver the highly accurate results.

 

Speaker

Hui Zhao
Pre-Sales Application Engineer
Agilent Technologies, Inc.

 

Hui has 13 years of industry experience developing and validating analytical methods for food/feed nutrition, food safety, dietary supplements and botanicals testing, using a variety of analytical techniques including LC-TQ, LC-QTOF, LC-DAD and GC-MS and a breadth of sample preparation methodologies. She has worked as a Research Scientist at Monsanto, EPL-Bioanalytical Services, Tate & Lyle, Inc. and Lead Staff Scientist at Covance Food Solutions. She holds a Master of Science degree in Analytical Chemistry from Lanzhou University in China and a Ph.D. in Analytical Chemistry from the University of Missouri.

Prior to becoming a Pre-Sales AE, Hui worked as an LCMS application scientist in Agilent’s global market development group where her primary focus was food and environmental market development.

 

Register and watch on demand >

 

Aqueous Film Forming Foam Formulations

Aqueous film forming foam (AFFF) is an effective fire suppressant for petroleum-based fires.  Foams are primarily composed of complex mixtures of per- and polyfluorinated substances (PFAS), but the exact composition is protected business information.  Identification of PFAS using High–resolution mass spectrometry (HR-MS) only utilizes the accurate mass and isotope pattern of the molecular ion and is not robust as formulas do not provide structural information.  Fragment ions from MSMS spectra can greatly improve identification confidence with software tools such as Fluoromatch. Data-dependent acquisition (DDA) is a common tool used to acquire MSMS spectra when the composition is unknown.  In this study, three approaches based on DDA acquisition for the MSMS fragmentation of fluorinated compounds were optimized and compared.

 

Speaker

Emily Parry, PhD
LC/MS Applications Scientist
Agilent Technologies, Inc.

 

Emily Parry received her PhD degree in Environmental Chemistry from the University of California, Davis. She joined Agilent after completing her postdoctoral work at the Environmental Protection Agency (EPA). Her specialty is method development and measurement of emerging contaminants.

 

Register and watch on demand >

 

Dioxins Analysis in the Environment

U.S. EPA Method 1613B has been one of the primary methods used in the analysis of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in wastewater, soils, sludges, and other matrices. These compounds have more than 200 congeners, and seventeen of them are highly toxic and of interest in trace analysis. Historically, these analyses have been performed using magnetic sector high-resolution GC/MS. In 2021, the U.S. EPA approved an alternate testing protocol (ATP) that accepts GC/TQ as an equivalent technology for the analysis of dioxins in environmental samples.  This work shows the Agilent triple quadrupole GC/MS system can achieve the sensitivity required to meet and exceed guidance for the analysis of PCDD/PCDFs at all concentration levels.

 

Speaker

Joel Ferrer
Product Manager – Agilent QQQ GC/MS
Agilent Technologies, Inc.

 

Joel Ferrer is the Product Manager for the Agilent Triple Quadrupole GC/MS portfolio. He obtained his B.S. in Biomedical Sciences at Texas A&M University where he gained experience on Agilent LC/MS and GC/MS instrumentation performing metabolomics research in the Department of Chemistry. Joel later earned his MBA from the University of Houston with a focus in Product Management and Marketing Analytics. He’s been with Agilent for over 5 years and in his current product management role since June 2020.

 

Register and watch on demand >

 

High-Throughput Intact Native Protein Analysis

Ion mobility-mass spectrometry has become a valuable analytical tool in native protein analysis. In protein structure studies, ion mobility spectrometry provides rotationally averaged collision cross-section values that correlate to size and shape of the biomolecule. For proteins, ground state CCS and accurate mass are not adequate to identify different proteins. Therefore, the introduction of gas phase unfolding followed by ion mobility measurements provide unique fingerprints for native protein analysis. This collision-induced unfolding (CIU) technique can be utilized to identify proteins and protein complexes. Typical CIU experiments utilize static nano-ESI or standard ESI using a syringe pump for sample introduction which is difficult to automate. In this study, we have developed a new automated sample introduction method for high-throughput CIU experiments which can be adapted for IgG and other proteins.

 

Speaker

Sheher Banu Mohsin, Ph.D.
Senior Applications Scientist
Agilent Technologies, Inc.

 

Sheher Mohsin is a senior applications scientist at Agilent Technologies. She received her Ph. D in physical chemistry from the University of Illinois and an MBA from Rockhurst University. She started her career at the US Environmental Protection Agency working on dioxin analysis with high-resolution mass spectrometers. She later joined Bayer and worked in the special analysis lab using mass spectrometry to solve problems in synthesis, impurity determination and submission of final product impurity profile to regulatory agencies. Sheher’s current focus is on lipidomics using GC, LC and SFC separations and mass spectrometry. Sheher collaborates with academic and government researchers working on complex problems to come up with innovative, simplified workflows using the latest tools in separation and mass spectrometry.

 

Register and watch on demand >