Tag: Analysis

Analysis of Nitrosamine Impurities in Pharmaceuticals

What you will learn

  • Understand what mutagenic impurities are and why it’s important to characterize and quantify them, even when present in trace amounts.
  • Understand the current regulatory guidelines for nitrosamines analysis in drug substance and product in Angiotensin II Receptor Blockers (sartan), ranitidine and metformin drugs
  • Learn about approaches for the prevention of the formation of GTIs and the confident identification and quantification of nitrosamines in APIs and drugs

 

Who should attend

  • Laboratory managers
  • Chromatographers
  • Analytical chemists and scientists
  • New product developers
  • Pharma manufacturing and quality control managers

 

Speaker

Raman V.V.S.S. Nanduri, Ph.D.
Director,
Analys Lab Pvt. Lt

 

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Sweetening your Sugar Analysis with Electrichemical Detection

In this talk we compare three commonly used techniques for the analysis of sugars. We will show the integration of the Metrohm Electrochemical detector into the Agilent OL CDS software. We will demonstrate the LOD and LOQs of each detector for a series of standards and samples.

 

Speaker

Sue D’Antonio
Applications Scientist
Agilent Technologies, Inc.

 

Sue D’Antonio joined Agilent (HP at that time) more than 30 years ago. For the last 15 years Sue has been an Applications Scientist in the Liquid Phase Separations Group. Sue’s application support extends to HPLC, UHPLC, CE and LCMS across a wide range of markets and customers.

 

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Dioxins Analysis in the Environment

U.S. EPA Method 1613B has been one of the primary methods used in the analysis of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) in wastewater, soils, sludges, and other matrices. These compounds have more than 200 congeners, and seventeen of them are highly toxic and of interest in trace analysis. Historically, these analyses have been performed using magnetic sector high-resolution GC/MS. In 2021, the U.S. EPA approved an alternate testing protocol (ATP) that accepts GC/TQ as an equivalent technology for the analysis of dioxins in environmental samples.  This work shows the Agilent triple quadrupole GC/MS system can achieve the sensitivity required to meet and exceed guidance for the analysis of PCDD/PCDFs at all concentration levels.

 

Speaker

Joel Ferrer
Product Manager – Agilent QQQ GC/MS
Agilent Technologies, Inc.

 

Joel Ferrer is the Product Manager for the Agilent Triple Quadrupole GC/MS portfolio. He obtained his B.S. in Biomedical Sciences at Texas A&M University where he gained experience on Agilent LC/MS and GC/MS instrumentation performing metabolomics research in the Department of Chemistry. Joel later earned his MBA from the University of Houston with a focus in Product Management and Marketing Analytics. He’s been with Agilent for over 5 years and in his current product management role since June 2020.

 

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Optimised Analysis of SVOCs with EPA 8270E

This webinar will overview a sensitive method used to analyse semivolatile organic compounds (SVOCs) on an Agilent 7000E Gas Chromatograph/Triple Quadrupole (GC/TQ) Mass Spectrometer. The utilization of GC/TQ instrumentation for analysis of SVOCs offers significant advantages:

  • Excellent selectivity afforded by Multiple Reaction Monitoring (MRM) mode results in faster batch review and increased confidence due to the elimination of matrix interferences often present when using GC/MS acquisition modes; and
  • Better sensitivity enables smaller extraction volumes which improves sustainability, reduces waste, and decreases costs associated with sample preparation, solvent usage, and waste disposal.
  • Key analytical techniques which facilitated this work will be discussed.
  • Manufacturer’s recommended tune protocol for mass accuracy and resolution checks in accordance with US EPA Method 8270E will also be discussed.

 

Speaker

Rachael Ciotti
GC/MS Application Specialist
Agilent Technologies, Inc.

 

Rachael Ciotti is a GCMS Application Specialist at Agilent Technologies in Wilmington, DE. Previously, she was a Field Service Engineer specializing in installing, maintaining, and repairing Agilent GC and GC/MS systems. Prior to joining Agilent, Rachael worked at DuPont as an analytical chemist responsible for GC and GC/MS method development and transfer to manufacturing labs. She holds a Bachelor of Arts in Mathematics from Rutgers University.

 

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Knowing How to Make ICP-MS Easier

Knowing the right information at the right time can help you cut through the everyday complexities of your ICP-MS analysis.

Driven by insights from customers from around the world, Agilent has developed a range of Easy-fit supplies, intuitive yet powerful software and complete workflows to bring the confidence of knowing you will get the answers you need to efficiently run your lab.

Watch the presentation and learn how to make your ICP-MS applications easier to run, maintain and control.

 

What you will learn

  • Learn how our range of Easy-fit supplies can simplify your workflow
  • Discover our selection tools that can help you get your ICP-MS configured right for your analysis
  • Take a look at ICP-MS MassHunter software
  • Learn about some of the simple software tools and time-saving tips that can help you get the very best out of your ICP-MS.

 

Speakers

Gareth Pearson
ICP-MS Supplies Product Manager (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia)

Angie is a proteomics scientist on the application team at Evosep BioSciences. Her work focuses on bringing standardization, throughput, and depth into translation proteomic research studies. During her PhD at University College Dublin studying proteomics in inflammatory arthritis and postdoc at Cedars Sinai, Los Angeles, Angie conducted large scale biomarker studies, where she gained considerable experience with the Evosep One and the Agilent triple quadrupole mass spectrometers.

 

Gareth Pearson
ICP-MS Supplies Product Manager (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia)

Angie is a proteomics scientist on the application team at Evosep BioSciences. Her work focuses on bringing standardization, throughput, and depth into translation proteomic research studies. During her PhD at University College Dublin studying proteomics in inflammatory arthritis and postdoc at Cedars Sinai, Los Angeles, Angie conducted large scale biomarker studies, where she gained considerable experience with the Evosep One and the Agilent triple quadrupole mass spectrometers.

 

Gareth Pearson
ICP-MS Supplies Product Manager (Agilent, Spectroscopy Technology Innovation Centre, Melbourne, Australia)

Angie is a proteomics scientist on the application team at Evosep BioSciences. Her work focuses on bringing standardization, throughput, and depth into translation proteomic research studies. During her PhD at University College Dublin studying proteomics in inflammatory arthritis and postdoc at Cedars Sinai, Los Angeles, Angie conducted large scale biomarker studies, where she gained considerable experience with the Evosep One and the Agilent triple quadrupole mass spectrometers.

 

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Novel Front-end Strategies to Increase Sample Throughput in LC/MS

Imagine if you could perform ‘LC/MS’ analysis in a comparable time to Usain Bolt’s 100-metre world record!  Over the last 15 years through iterative technological product enhancements and by the replacement of a traditional UHPLC with a simple sample cleanup step, the RapidFire system has accelerated MS analyses in many fields ranging from HTS, ADME, biopharma, synthetic biology, food, and the life sciences.

However, sometimes when there is an unavoidable requirement for good chromatographic separation, then an alternative, low-cost strategy exploiting Ultra-Fast LC can be adopted to reduce analysis times from minutes to seconds. This webinar presents the new RapidFire400 system for HTS and will contrast its analytical merits to the complimentary Ultra-Fast LC approach.

 

What you will learn

•    How the RapidFire400 meets the needs of  today’s high throughput requirements
•    Learn how a standard Agilent LC can be configured to the Ultra-Fast LC mode
•    Understand the advantages and limitations of both high sample throughput strategies

 

Speaker

Andreas Mielcarek
Pre-Sales Applications Scientist
Agilent

 

Andreas Mielcarek is a pre-sales Applications Scientist with Agilent Technologies. He has been with Agilent for 5 years developing LC, RapidFire, and mass spec methods on a wide range of molecules. Prior to joining Agilent, he worked as a LC-MS Core-facility scientist at the University Marburg, were he obtained his PhD in bioanalytical chemistry.

 

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Improved Metabolomics Analysis Using an Iron-Free Flow Path

Metabolomics is a tool to decipher and understand the physiological state of a cell or organism. In recent times, LC/MS emerged as the prevalent analytical technique of choice, still with significant potential for improved robustness and ease of use.

In this webinar, we will demonstrate the advantages gained by using a biocompatible flow path over stainless steel by direct comparison of the Agilent 1290 Infinity II Bio LC and Agilent 1290 Infinity II LC. The 1290 Bio LC showed improved peak shape and resolution for phosphorylated compounds like nucleotides and sugar phosphates without apparent adsorption effects, compared to the Agilent 1290 Infinity II LC. Analyzing intracellular metabolite extracts derived from Saccharomyces cerevisiae, excellent retention time RSD values of 0.1% were generated, and essential metabolites for physiological parameters like the adenylate energy charge could be analyzed consistently. These results show that the 1290 Bio LC is the ideal choice for the seamless and robust analysis in metabolomics.

 

 What you will learn

•    How HILIC/MS separation is used for the detection of metabolites.
•    How an iron-free flow path delivers superior performance for phosphorylated metabolites.
•    Improved tailing factors of nucleotides
•    Robust performance in analyzing metabolites in a complex yeast extract

 

Speaker

Andre Feith
Application Scientist
Agilent Technologies

 

André Feith is an HPLC application scientist at Agilent Technologies, focusing on biopharma and biotechnological topics. He was involved in testing and validation of the Agilent 1290 Infinity II Bio LC regarding bio-specific applications and more recently he has focused on analyzing new modalities like oligonucleotides and online analysis of critical quality attributes with process analytical technology (PAT). During his Ph.D., he researched at the Institute of Biochemical Engineering, University of Stuttgart, in the field of Bioanalysis, Metabolomics, and Metabolic Engineering.

 

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