Category: Upcoming and New Events

Improved Metabolomics Analysis Using an Iron-Free Flow Path

Metabolomics is a tool to decipher and understand the physiological state of a cell or organism. In recent times, LC/MS emerged as the prevalent analytical technique of choice, still with significant potential for improved robustness and ease of use.

In this webinar, we will demonstrate the advantages gained by using a biocompatible flow path over stainless steel by direct comparison of the Agilent 1290 Infinity II Bio LC and Agilent 1290 Infinity II LC. The 1290 Bio LC showed improved peak shape and resolution for phosphorylated compounds like nucleotides and sugar phosphates without apparent adsorption effects, compared to the Agilent 1290 Infinity II LC. Analyzing intracellular metabolite extracts derived from Saccharomyces cerevisiae, excellent retention time RSD values of 0.1% were generated, and essential metabolites for physiological parameters like the adenylate energy charge could be analyzed consistently. These results show that the 1290 Bio LC is the ideal choice for the seamless and robust analysis in metabolomics.

 

 What you will learn

•    How HILIC/MS separation is used for the detection of metabolites.
•    How an iron-free flow path delivers superior performance for phosphorylated metabolites.
•    Improved tailing factors of nucleotides
•    Robust performance in analyzing metabolites in a complex yeast extract

 

Speaker

Andre Feith
Application Scientist
Agilent Technologies

 

André Feith is an HPLC application scientist at Agilent Technologies, focusing on biopharma and biotechnological topics. He was involved in testing and validation of the Agilent 1290 Infinity II Bio LC regarding bio-specific applications and more recently he has focused on analyzing new modalities like oligonucleotides and online analysis of critical quality attributes with process analytical technology (PAT). During his Ph.D., he researched at the Institute of Biochemical Engineering, University of Stuttgart, in the field of Bioanalysis, Metabolomics, and Metabolic Engineering.

 

Register and watch on demand >

 

SFC – Only the Solvent is Critical

Supercritical Fluid Chromatography (SFC) was first developed in the 1960s, using supercritical carbon dioxide and this technology has been commercially available from Agilent for over a decade. Often known for its application to the analysis of chiral compounds, SFC is the ‘greener’ alternative over normal phase separations, further increasing the demand and acceptance of this technology.

This webinar will explain the basic scientific principles of SFC as a highly powerful liquid separation technique for the efficient separation a wide range of analytes. Examples will be given to illustrate, how an SFC system can be coupled to a mass detector to form an integrated LC/MS system to deliver high throughput applications.

 

 What you will learn

•    Similarities and differences between SFC and conventional HPLC
•    Why SFC is the green technique in separation sciences.
•    The advantages of SFC with application examples
•    Theoretical background to SFC
•    How to set up an SFC system with MS detection

 

Speaker

Dr. Jens Trafkowski
Product Specialist HPLC
Agilent Technologies

 

Jens Trafkowski studied food chemistry and received his PhD at the Institute of Legal Medicine from the University of Bonn for his work on applications of HPLC-MS/MS in forensic and clinical toxicology.

Prior to joining Agilent in 2011, Jens spent more than 6 years as LC-MS as an application specialist in the industry before leaving to undertake a global HPLC product management responsibility at Agilent Technologies. Later, in 2017 Jens moved into his current role as an HPLC product specialist where he is based in Basel Switzerland.

 

Register and watch on demand >

 

Boost your Performance with 2D-LC Separation

This scientific presentation is for analytical chemists, chromatographers, and mass spectrometrists that want to learn, how an additional chromatographic dimension can deliver deeper insights into their samples, particularly when integrated with a mass selective detection system.

Several concepts of the different types of 2D-LC will be explained to enable a comprehensive understanding of this well-established technology.

The benefits of comprehensive Multiple Heart-Cutting, High-Resolution Sampling 2D-LC and the unique technique of Active Solvent Modulation will be explained in detail. Real-life examples will be shown to illustrate the advantages that incorporating an additional separation dimension brings to LC and LC/MS analysis.

 

What you will learn

•    First principles of 2D-LC technology
•    How to configure a mass detector with 2D-LC
•    How 2D-LC technologies enhance the quality of MS detection
•    Where 2D-LC makes a positive difference with several application examples

 

Speaker

Dr. Jens Trafkowski
Product Specialist HPLC
Agilent Technologies

 

Jens Trafkowski studied food chemistry and received his PhD at the Institute of Legal Medicine from the University of Bonn for his work on applications of HPLC-MS/MS in forensic and clinical toxicology.

Prior to joining Agilent in 2011, Jens spent more than 6 years as LC-MS as an application specialist in the industry before leaving to undertake a global HPLC product management responsibility at Agilent Technologies. Later, in 2017 Jens moved into his current role as an HPLC product specialist where he is based in Basel Switzerland.

 

Register and watch on demand >

 

Customer Stories | Jimmy Woodley

How many GCs can one lab need? In all the years Chemetrix has been supplying top-quality instruments to labs across the country, there’s one customer who surprised us with an ambitious request.

Jimmy Woodley is the now retired lab manager of the chromatography division of Sasol in Secunda. He guided the division through a period of incredible growth and, when he decided to take his team to the next level, he trusted Chemetrix to help equip his lab with Agilent GCs to set them far apart from any other operation in South Africa.

By the time Jimmy retired, his division was working with 160 dual channel GCs and, at a stage, he had over 40 Agilent GCs in a single order. This was a customer relationship that spanned years and Chemetrix had the honour of working with Jimmy and his team to drive the growth of their analysis as well as enhance their capabilities as a laboratory.

Watch Jimmy tell the story in his own words and reflect on a fond relationship with the team at Chemetrix.

Chemetrix Backs Yes 4 Youth

SA Youth is supported by the Presidency, YES, National Youth Development Agency (NYDA) and Harambee Youth Employment Accelerator as part of the Presidential Youth Employment Intervention.

The initiative is explained best by the pledge that we are signatories to.

“Through my company’s participation in the YES initiative, I hereby register my organisation with the Youth Employment Service (RF) NPC (registration number 2017/267641/08). I agree with the CEO pledge, acknowledging the critical role that youth play in shaping our economy and our country. I acknowledge that my participation in the programme is giving youth their first chance at a work opportunity and I undertake, as far as possible, to ensure youth are nurtured and encouraged in their placement.

Together with my organisation I am committed to being a co-creator in a future that works for all South Africans, with an understanding that we are subscribing to the values and philosophy of YES. This is based on humanitarian development and socio-economic principles, in the building of a pathway to guide and empower young unemployed South Africans into economic participation. Given the scale and implications of South Africa’s youth unemployment situation, the country and YES must succeed together.”

Chemetrix believes that, through our participation in this program, we are doing our bit however small it may be, to be better corporate citizens. Through this program Chemetrix has an opportunity to not only provide job opportunities, but also provide much needed skills to equip our youth for the future.

 

We are very proud to announce that we have appointed no less than 11 youth for the 2022 calendar year:

Collen Tshakhosi
Warehouse Clerk Trainee

Thulani Hlatshwayo
Warehouse Clerk Trainee

Rachel Londanie
General Cleaner

Boipelo Motsamai
Transactional Sales Intern

Ongezwa Mangindana
Transactional Sales Intern

Nompumelelo Tamara Ndelu
Transactional Sales Intern

Tankiso Lawrence Ngake
Customer Technical Support Trainee

Thembelihle Nkosi
Sales Office Assistant

Andiswa Msomi
Transactional Sales Intern

Samukelisiwe Shabalala
Trainee Administrative Clerk

Ipeleng Rakgotla
Computer Support Assistant