Tag: webinar

CE/MS: From Small Ions to Biomolecules – Think CE!

Capillary Electrophoresis is extremely well suited to the separation of charged molecules whether they are small pesticide metabolites (<110 Da)  or large biomolecules such as whole proteins and antibody-based drugs (>150KDa). The technique is characterized by high-efficiency separations, allowing high peak capacity, and speed, only requiring very small sample volumes (ca. 10ul).

When coupled with Mass Spectrometry, CE/MS provides maximum separation power and sensitivity for compound identification, particularly when coupled to High-Resolution QTOF MS instrumentation. This webinar will provide a direct comparison of CE with LC separations and will highlight areas of complementarity and orthogonality. With examples of analyses ranging from small pesticide metabolites to antibody-drug investigations and specialized isoelectric focusing applications (cIEF), this webinar will demonstrate the true power of CE/MS in a modern laboratory.

 

 What you will learn

•    When to use CE separations over UHPLC
•    How to couple CE to MS
•    Why CEMS is Complementary to LCMS.
•    How cIEF can be used with mass spectrometry

 

Speaker

Gordon Ross
Application Scientist
Agilent

 

Gordon has worked in the field of separation sciences using CE and LC since the 1980’s and with mass spectrometry for 25 years. He has been with Agilent for 28 years and is currently a Senior Applications Scientist. He has worked in many analytical areas from metabolomics to environmental analysis and has interests in Target, Suspect and Non-Target Screening using High-Resolution Mass Spectrometry.

 

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Improved Metabolomics Analysis Using an Iron-Free Flow Path

Metabolomics is a tool to decipher and understand the physiological state of a cell or organism. In recent times, LC/MS emerged as the prevalent analytical technique of choice, still with significant potential for improved robustness and ease of use.

In this webinar, we will demonstrate the advantages gained by using a biocompatible flow path over stainless steel by direct comparison of the Agilent 1290 Infinity II Bio LC and Agilent 1290 Infinity II LC. The 1290 Bio LC showed improved peak shape and resolution for phosphorylated compounds like nucleotides and sugar phosphates without apparent adsorption effects, compared to the Agilent 1290 Infinity II LC. Analyzing intracellular metabolite extracts derived from Saccharomyces cerevisiae, excellent retention time RSD values of 0.1% were generated, and essential metabolites for physiological parameters like the adenylate energy charge could be analyzed consistently. These results show that the 1290 Bio LC is the ideal choice for the seamless and robust analysis in metabolomics.

 

 What you will learn

•    How HILIC/MS separation is used for the detection of metabolites.
•    How an iron-free flow path delivers superior performance for phosphorylated metabolites.
•    Improved tailing factors of nucleotides
•    Robust performance in analyzing metabolites in a complex yeast extract

 

Speaker

Andre Feith
Application Scientist
Agilent Technologies

 

André Feith is an HPLC application scientist at Agilent Technologies, focusing on biopharma and biotechnological topics. He was involved in testing and validation of the Agilent 1290 Infinity II Bio LC regarding bio-specific applications and more recently he has focused on analyzing new modalities like oligonucleotides and online analysis of critical quality attributes with process analytical technology (PAT). During his Ph.D., he researched at the Institute of Biochemical Engineering, University of Stuttgart, in the field of Bioanalysis, Metabolomics, and Metabolic Engineering.

 

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SFC – Only the Solvent is Critical

Supercritical Fluid Chromatography (SFC) was first developed in the 1960s, using supercritical carbon dioxide and this technology has been commercially available from Agilent for over a decade. Often known for its application to the analysis of chiral compounds, SFC is the ‘greener’ alternative over normal phase separations, further increasing the demand and acceptance of this technology.

This webinar will explain the basic scientific principles of SFC as a highly powerful liquid separation technique for the efficient separation a wide range of analytes. Examples will be given to illustrate, how an SFC system can be coupled to a mass detector to form an integrated LC/MS system to deliver high throughput applications.

 

 What you will learn

•    Similarities and differences between SFC and conventional HPLC
•    Why SFC is the green technique in separation sciences.
•    The advantages of SFC with application examples
•    Theoretical background to SFC
•    How to set up an SFC system with MS detection

 

Speaker

Dr. Jens Trafkowski
Product Specialist HPLC
Agilent Technologies

 

Jens Trafkowski studied food chemistry and received his PhD at the Institute of Legal Medicine from the University of Bonn for his work on applications of HPLC-MS/MS in forensic and clinical toxicology.

Prior to joining Agilent in 2011, Jens spent more than 6 years as LC-MS as an application specialist in the industry before leaving to undertake a global HPLC product management responsibility at Agilent Technologies. Later, in 2017 Jens moved into his current role as an HPLC product specialist where he is based in Basel Switzerland.

 

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