High-throughput LC-MS Analysis to Systematically Map Gut Microbiome Drug Metabolism

Individuals vary widely in their drug responses, which can be dangerous and expensive due to significant treatment delays and adverse effects. Growing evidence implicates the gut microbiome in this variability, however the molecular mechanisms remain mostly unknown.

To systematically map the drug metabolizing capacity of the gut microbiota and assess its potential contribution to drug metabolism, the speaker measured the ability of 76 diverse human gut bacteria to metabolize each of 271 oral drugs. They found that two-thirds of these drugs are chemically modified by at least one of the tested microbes.

Through combination of high-throughput bacterial genetics with mass spectrometry-based metabolomics, the speaker systematically identified drug-metabolizing microbial enzymes. These proteins better explain the drug metabolizing capacity of bacterial strains than their phylogenetic classification.

The speaker further demonstrated that the abundance of homologs of these proteins predict the capacity of complete human gut communities to metabolize the targeted drugs. These causal links between microbiota gene content and metabolic activities connect inter-individual microbiome variability to interpersonal differences in drug metabolism, which has translatable potential on medical therapy and drug development across multiple disease indications.

 

Learn how to:

  • Employ high-throughput LC-MS analysis to map gut microbial metabolism
  • Combine bacterial genetics with high-throughput LC-MS analysis to identify metabolic enzymes in the gut microbiome
  • Integrate in vitro drug metabolism of gut bacteria, genomic information, and metagenomic data.

 

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Keeping Pace with COVID-19 Virtual Symposium

COVID-19 research and testing methods are evolving every day. Join us for an interactive live event to hear the latest on what leading researchers and labs are doing to keep pace.
You will discover best practices for applying technology to support COVID-19 research, test development, and vaccine and drug development – and how these workflows can be effectively applied toward other infectious diseases as well.

 

Register to access a diverse lineup of speakers and sessions, including:

  • Collaborating in the fight against COVID-19
  • A next-generation tool bench for virology research
  • Keynote presentation: Viral hijacking of cellular metabolism
  • Seahorse XF reveals bioenergetic impact in virology
  • Rapid functional evaluation of virus-neutralizing antibodies and antiviral drugs using multiparametric live-cell analysis
  • Focus on virology: Applications to enable discovery
  • SLIMS: Easier and faster LIMS deployment for COVID
  • Faster in, faster out: Achieve the most efficient analysis of raw material for vaccine production
  • Agilent BioMDS solutions for vaccine research
  • Agilent GC solution for alcohol-based hand sanitizer testing
  • Analysis of alcohol levels in hand sanitizer formulations using Cary 630 FTIR
  • QA/QC of protection masks

 

Agenda

 

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