• Affordable, benchtop confocal imaging for every laboratory.
• Confocal microscopy enables improved image quality and analysis, revealing a level of detail in samples that is not possible with widefield optics.
• High-quality optical components, including the Hamamatsu sCMOS Orca camera, Semrock filters, Olympus objectives allow the capture of stunning, publication-quality images.
• Water-immersion objectives to capture more light with lower exposure times.
• Combination of spinning disk confocal and widefield imaging plus multimode reader, allows the Cytation C10 to be ready for any assay.
• An available 60 µm deep sectioning disk (DSD) allows a clear view into thicker samples.
• Widefield imaging allows for faster acquisition of large samples at lower magnification, while confocal images small intracellular details or 3D samples, and both modes together provide multiparameter imaging experiments.
• Environmental controls, including incubation and CO₂/O₂ control and monitoring, facilitate live cell imaging.
• Imaging plus multimode detection and the hit-picking function quickly pre-screen the microplate with the plate reader optics, then automatically image the samples that meet your hit criteria, saving time and data storage capacity.

Imaging

• 3D cell culture
• Cell viability/toxicity
• Cell migration
• Automated ROI identification
• Time-lapse live cell imaging
• Label-free cell counting
• Slide scanning
• Whole-organism imaging
• Cell cycle analysis
• Calcium kinetics
• Transfection efficiency
• Stem cell differentiation
• Genotoxicity

Multimode detection

• ELISA
• Nucleic acid and protein quantification
• Luciferase reporter assays
• Cell growth studies

• Sophisticated imaging with both upright and inverted microscopy optics offers a range of cellular and reflected light applications, yielding information in many imaging modes.
• Inverted microscopy module supports fluorescence, brightfield, and color brightfield to analyze both large objects and intracellular detail and enables a broad range of applications.
• Combination of hybrid plate reader and advanced microscopy mode provides flexible operation ready for any assay, saves time, and increases productivity in the laboratory.
• Multimode detection plus imaging saves time and data storage
• Automation of imaging acquisition, processing, image analysis, and object count reduces manual intervention.
• Gen5 software facilitates powerful ROI identification, samples may be selected for high magnification and the process of imaging ROIs in batches can be accelerated for faster throughput.
• Take3 plate or Take3 Trio allows analysis of 16 or 48 samples in a single run, saving time compared to single-sample devices.
• Increased sensitivity with large bandwidth settings and increased specificity with small bandwidth settings enhance assay performance and flexibility for applications.

Imaging

• LD cell imaging
• Cell migration/invasion assays
• ELISpot assay imaging
• Slide scanning
• Live cell imaging
• Cell viability/toxicity
• Colony counting

Multimode detection

• ADME/Tox assays
• ELISA
• Nucleic acid and protein quantification
• Inject/read calcium assays
• ROS assays
• Flash luminescence assays
• Cell growth assays

• Modular, upgradable imaging modes, plus multimode detection enables a range of cell-based assays and applications beyond a standard microplate reader.
• Combination of hybrid plate reader and advanced microscopy mode increases productivity for a system that is ready for any assay.
• Hit-picking capability reduces time and computer memory with no unnecessary data collection or analysis
• Fully automated microscopy tasks, from slide scanning to time-lapse live cell assays, and flexible hardware increase productivity and reduce manual labor.
• Combination of monochromator and filter optics delivers flexibility and performance to microplate assays, and choice of optics allows for a wide range of applications.
• BioTek Gen5 software enables walk-away automation for your image process and analysis workflows and data analysis tasks.
• Variable bandwidth quad monochromator optics enhance assay performance including increased sensitivity and lower limits of detection.

Imaging

• Label-free cell counting
• Calcium kinetics
• Time-lapse live cell imaging
• 3D cell culture
• Slide scanning
• Cell viability/toxicity assays
• Cell migration assays
• Stem cell differentiation
• Genotoxicity

Multimode detection

• ELISA
• TR-FRET
• Luciferase Reporter assays
• Nucleic acid and protein quantification
• AlphaScreen
• Fluorescence polarisation

• Digital microscopy and multimode detection deliver both phenotypic data and quantitative data from one instrument, maximizing laboratory productivity. Gen5 software provides image and data analysis for all applications in an easy to use package.
• Easy imaging workflow automation with augmented microscopy image capture, processing, and analysis. Automated results as publication-ready images and data save time and reduce the need for manual manipulation and transfer to other applications.
• Fluorescence and high contrast brightfield imaging from 1.25x to 60x to capture large regions of interest and intracellular details.
• Automated XY stage, autofocus, auto exposure, and auto LED intensity provide efficient capture of high-quality images in an affordable package.
• More than 20 filter/LED color cubes available for a large selection of colors. This comprehensive selection of imaging filter and LED.

Imaging

• 2D and 3D cell imaging and analysis
• Cell proliferation studies
• Label-free cell counting
• Cytotoxicity
• Biomarker quantification

Multimode detection

• Drug discovery
• Genetic analysis
• Drug absorption and metabolism
• Biologics drug discovery and development
• Environmental testing
• Food safety
• Nucleic acid quantification
• Protein quantification

• Five imaging modes within a single, flexible, compact system. Automate workflows with a variety of imaging modes that enable a full range of microscopy applications in microplates, cell culture dishes, flasks, and other labware.
• Broad magnification range allows for detailed image capturing, from whole well montages to intracellular detail at 1.25x to 60x. The automated, 6-position turret makes it simple to capture multiple magnifications in a single workflow.
• Environmental controls in BioSpa integrate temperature and CO₂/O₂ control and monitoring, plus humidity monitoring. Stable conditions keep cells healthy, aid in proliferation, and protect cells.
• Automated 6-objective turret provides efficient viewing of a variety of samples at a wide range of magnifications. Capture images automatically at one or more magnifications, within a single experiment.
• 3D cell culture analysis with Gen5 software enabled by powerful z-stacking capabilities that capture the important details of 3D cell structure. Z-projection and image analysis and z-stacking montage capability bring powerful processing for 3D biologies.
• With the Agilent BioTek Cytation cell imaging multimode reader integrated, a laser autofocus method delivers increased reproducibility and speed, while decreasing phototoxicity and photobleaching.
• Advanced cell-level analysis with powerful tools like nuclear and cytoplasmic masking. These tools increase efficiency by defining critical, information-rich regions of interest for automated, detailed cellular analysis.
• Compact design allows the system to fit inside biosafety cabinets. Partners such as The Baker Company offer a broad range of innovative solutions to provide isolation of cell lines and containment of potentially hazardous agents.

• Cell migration and invasion
• Cell cycle progression and analysis
• Cytotoxicity
• Apoptosis and necrosis assays
• Cell proliferation assays

• Small footprint for easy installation on most standard lab benches.
• No external instrumentation or dedicated darkroom is required for image capture.
• Wide field of view (WFOV) camera provides fast automated imaging in microplates and slides, enabling researchers to get the answers they need quickly.
• Gen5 imaging software offers unique, full automation to capture, process, analyze, and publish workflows.
• Environmental controls enable Lionheart FX to image and analyze live cell assays over time.
• Laser-based and software-based autofocus capture sharp, in-focus images in automated microscopes.
• Open design of the Lionheart FX stage allows researchers to work with microfluidic devices.
• Dual reagent injector module for Lionheart FX allows fast cellular reactions to be imaged after the addition of a reagent.

• 2D and 3D cell imaging and analysis
• Cell growth and death dynamics
• Label-free cell counting
• Cell viability/toxicity
• Immunofluorescence
• Subpopulation analysis
• Cell migration/invasion assays
• Wound healing

• xCELLigence RTCA MP model can run up to six 96-well plates simultaneously or independently, with flexible plate batch processing to maximize productivity for multiple users.
• MP system measures cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility.
• RTCA MP analyzer obtains continuous data at high temporal resolution (from seconds to days), with non-invasive and label-free real-time impedance technology.
• Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time.
• Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50.
• The instrument operates in a standard CO₂ cell culture incubator and the control unit is housed outside the incubator.

• Cell health characterization
• Immune cell-mediated killing
• Viral cytopathic effects
• Cytotoxicity, cell adhesion
• Cell barrier function (e.g. TEER)
• Cell signalling (e.g. GPCR)

• xCELLigence RTCA DP model can run up to three separate electronic 16-well plates (E-Plate 16 or CIM-Plate 16).
• Each well in the CIM-Plate 16 is an electronically integrated Boyden chamber, which enables the monitoring of cell invasion and migration in real-time (at second or minute intervals) without exogenous labels.
• Non-invasive and label-free real-time impedance assay using E-plate 16 measures cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility.
• Obtain continuous data at high temporal resolution (from seconds to days)
• Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time
• Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50.
• RTCA Software Pro supports FDA 21 CFR Part 11 compliance to ensure electronic data integrity.

• Cell Invasion and Migration Assays
• Cell Adhesion Assays
• Cell Barrier Function Assays
• Immune Cell Killing Assays
• Viral Cytopathic Effect Assays
• Cytotoxicity Assays
• Cell Signaling Assays
• Stem Cell Assays

• Agilent xCELLigence RTCA SP model can run one 96-well plate.
• Measure cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility.
• Obtain continuous data at high temporal resolution (from seconds to days), with non-invasive and label-free real-time impedance technology.
• Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time.
• Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50.
• RTCA Software Pro supports FDA 21 CFR Part 11 compliance to ensure electronic data integrity.

• Cell health characterization
• Immune cell-mediated killing assays
• Viral cytopathic effects assays
• Cytotoxicity assays
• Cell adhesion assays
• Cell barrier function assays(e.g. TEER)
• Cell signaling assays (e.g. GPCR)

• Obtain continuous cell assay time points during the entire experiment.
• RTCA provides a continuous quantitative readout of cell number, proliferation rate, cell size/shape, and cell-substrate attachment quality.

• Immune cell killing assays
• Viral cytopathic effect assays
• Cytotoxicity assays
• Cell adhesion assays
• Cell signaling assays
• Stem cell assays
• Cell barrier function assays

• Provides a high-throughput method for detecting functional cardiotoxicity effects on short- and long-term cardio beating activity and for general toxicity.
• Correlates findings with arrhythmogenic activity.
• Provides insight into compound mechanism of action.

• Cardiac Disease Modeling
• Cardio Safety Toxicology
• Cardio Drug Discovery
• CiPA (Comprehensive In Vitro Proarrhythmia Assay) Initiative

• Improve cell maturation status in 2-3 weeks.
• Enable precise control of the beating rate over different time durations.
• Improve cardiomyocyte response to inotropic compounds and sarcomeric structure.
• Scalable system allows for the simultaneous monitoring of multiple plates.
• Compatible with calcium measurements, high content microscopy, voltage sensitive dyes, plate readers, etc.

• Cardiac Disease Modeling
• Cardio Maturation
• Cardio Drug Discovery
• Cardio Safety Toxicology

• Combine label-free xCELLigence real time cell analysis technology with live cell imaging in red, green, and blue for increased confidence in your cell analysis and conclusions.
• Deliver physiologically relevant data with a simple workflow that adapts to differing experimental protocols while monitoring cell health, strength of adhesion, changes in morphology, proliferation, and cytolysis in primary cell cultures or standard tissue culture cell lines.
• Utilize flexible live cell imaging with brightfield capabilities, three fluorescence channels, a multitude of well plate formats, and the capability of user-defined schedules.
• Analyze a continuous readout of cell number, proliferation rate, cell size/shape, and cell-substrate attachment quality.

• Immune Cell Killing Assays
• Viral Cytopathic Effect Assays
• Cytotoxicity Assays
• Apoptosis and Proliferation Assays
• Phagocytosis Assays

• Live cell, real-time analysis of cellular energy metabolism platform in 96-well format. 
• Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates. 
• Excellent OCR precision at low rates, providing confidence when working with quiescent cell types, such as naïve T cells. 
• High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate. 
• Compatible with XF Pro M plate to reduce edge effect. 
• Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time. 
• Verified instrument performance to ensure intraplate and interplate data consistency. 
• Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42°C 
• Advanced Pro Controller software for easy assay protocol creation with user guided interface. 
• Data Quality View in Wave Pro software for automated flagging of outliers. 

• Phenotypic screening
• Testing many conditions at one time
• Dose-response studies
• OMICS functional confirmation
• Spheroid metabolism

• Live cell, real-time analysis of cellular energy metabolism platform in 24-well format.
• Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates.
• Large well size and transient microchamber to provide the capacity for larger and/or more metabolically active samples.
• Robust response with as few as 10,000 cells per well in the custom 24-well plate.
• Compatible with 3D study models such as islets and small organism (for example, zebrafish).
• Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time.
• Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C for compatibility with a variety of samples.
• Data files compatible with the web-based analytics tool, namely, Seahorse Analytics.

• Immunology Research (e.g. determining metabolic fitness of immune cells, measuring and modulating immune cell activation in real time and targeting metabolic pathways to improve immunotherapy design and performance)
• Cancer Research (e.g. rewiring metabolism to support rapid growth, understanding which nutrients fuel cancer cells and uncovering how metabolism impacts the tumor microenvironment)
• Toxicology Research (e.g. detecting mitochondrial liabilities among potential therapeutics, assessing the risk of mitochondrial toxicity in vitro with high specificity and sensitivity and determining the mechanism of action of mitochondrial toxicants)
• Obesity, diabetes, and metabolic disorders research (e.g. measuring functional effects of genetic changes to metabolic pathway components, examining nutrient use in healthy and diseased cell models and assessing fatty acid oxidation and glycolysis in different cell types)

• Live cell, real-time analysis of cellular energy metabolism platform in eight-well format.
• Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates.
• Excellent OCR precision at low rates, providing confidence when working with quiescent cell types, such as naïve T cells.
• High sensitivity that requires 3x fewer cells to achieve robust response, most suited for studies with limited biological materials when working together with XF HS miniplates.
• Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time.
• Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C.
• Web-based analytics tool, Seahorse Analytics, for streamlined data analysis.

• Optimization of experimental conditions before scaling up
• Cellular metabolism assays
• Immune metabolism assays
• Cancer metabolism assays

Consumable packet typically includes:

• FluxPaks
• Miniplates and Miniplate Carriers
• Assay Kits and Reagents
• Assay Media and More

• Powerful capabilities with up to 17 parameter detection with enhanced sensitivity and resolution.
• Intuitive, advanced data analysis capabilities for greater usability and automated instrument maintenance functions.
• Customizable sampling with 1 to 3 laser options, exchangeable filters, multiple sampling options, and flexible analysis formats.
• Enhanced usability with fixed optical alignment, 24-bit detection dynamic ranges and no need for PMT voltage adjustment, accurate volumetric-based cell counting, pressure sensors to monitor fluidic status in real-time, and automatic cleaning and decontamination processes.
• NovoExpress software is intuitive and easy to use. Flexible analysis templates and plotting tools save time and increase efficiency in data analysis.

• Apoptosis Assay
• Immunophenotyping
• Cell Proliferation
• Cytokine Detection
• Intracellular Protein Detection
• Cell Cycle Analysis