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    Specifications

    Agilent xCELLigence RTCA Dual Purpose (DP) Instrument 

    The Agilent xCELLigence RTCA DP instrument continuously measures cell invasion and migration(CIM) and monitors cell health, behavior, and function using label-free cellular impedance.

    The three cradles on the DP instrument enable three separate electronic 16-well plates to be controlled and monitored in parallel or independently of one another. This flexible plate batch processing allows maximum productivity for multiple users. The instrument operates in a standard CO2 cell culture incubator and the control unit is housed outside the incubator. Online data acquisition and offline data analysis are easy to perform using RTCA Software Pro.

    Features

    • xCELLigence RTCA DP model can run up to three separate electronic 16-well plates (E-Plate 16 or CIM-Plate 16).
    • Each well in the CIM-Plate 16 is an electronically integrated Boyden chamber, which enables the monitoring of cell invasion and migration in real-time (at second or minute intervals) without exogenous labels.
    • Non-invasive and label-free real-time impedance assay using E-plate 16 measures cell health, viability, or response to treatments with high sensitivity, accuracy, and reproducibility.
    • Obtain continuous data at high temporal resolution (from seconds to days)
    • Easy workflow allows users to simply add cells to E-plates and begin kinetic measurements at physiological conditions, with minimal hands-on time
    • Intuitive RTCA Software Pro allows for analysis of real-time data to generate diverse plot types and automatically calculate parameters such as % of cytolysis, IC50 or KT50.
    • RTCA Software Pro supports FDA 21 CFR Part 11 compliance to ensure electronic data integrity.

    Applications

    • Cell Invasion and Migration Assays
    • Cell Adhesion Assays
    • Cell Barrier Function Assays
    • Immune Cell Killing Assays
    • Viral Cytopathic Effect Assays
    • Cytotoxicity Assays
    • Cell Signaling Assays
    • Stem Cell Assays

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