Protein analysis Archives - Chemetrix https://chemetrix.co.za/tag/protein-analysis/ Powerful Partnership, Inspiring Insights Thu, 06 Feb 2025 08:50:31 +0000 en-US hourly 1 https://wordpress.org/?v=6.3.6 The Role of Multimode Microplate Readers in Modern Laboratories https://chemetrix.co.za/the-role-of-multi-mode-microplate-readers-in-modern-laboratories/ https://chemetrix.co.za/the-role-of-multi-mode-microplate-readers-in-modern-laboratories/#respond Mon, 17 Feb 2025 08:00:40 +0000 https://chemetrix.co.za/?p=6731 Continue reading "The Role of Multimode Microplate Readers in Modern Laboratories"]]> In today’s fast-evolving scientific landscape, versatility and efficiency are paramount. Multimode microplate readers are fast becoming a popular and effective choice for modern laboratories, enabling researchers to conduct a wide range of assays with precision and ease. From absorbance to luminescence beyond, these instruments streamline workflows, reduce operational costs, and enhance reproducibility. Available through Chemetrix, advanced multimode microplate readers provide researchers with cutting-edge technology tailored to diverse applications, such as nucleic acid quantification, protein assays, and enzyme kinetics.

Understanding Multimode Microplate Readers

At their core, multimode microplate readers integrate several detection modes into a single device. This capability eliminates the need for multiple instruments, thereby saving space and resources while expanding a laboratory’s functional capacity.

Unlike single-mode readers, which are limited to one detection method, multimode readers can perform absorgance, fluorescence, luminescence, and time-resolved fluorescence measurements, among others. This versatility makes them ideal for high-throughput screening and routine laboratory applications.

 

Key applications

Nucleic acid quantification

Accurate nucleic acid measurement is critical in molecular biology and genomics research. Multimode readers facilitate DNA and RNA quantification through fluorescence and absorbance assays, ensuring reliable data for downstream applications such as sequencing or PCR.

Protein analysis

Protein quantification and kinetic studies are central to biochemistry and pharmaceutical research. Multimode readers enable assays like the Bradford or BCA assay, utilising absorbance for robust and repeatable results. Additionally, fluorescence-based protein-binding assays provide insights into protein interactions and conformational changes.

Enzyme kinetics

Enzymes are essential to numerous biological processes and industrial applications. Multimode readers are equipped to perform time-resolved fluorescence and luminescence measurements, offering real-time monitoring of enzymatic reactions. These capabilities help researchers study enzyme activity under various conditions and evaluate inhibitors or activators.

Cell viability and toxicity

In drug discovery and toxicology, understanding cell viability is crucial. Multi-mode readers support assays like MTT and ATP assays, providing critical data on cell health and metabolic activity.

📚 Download the Microplate-Based Cell Viability Assays Using Absorbance, Fluorescence, or Luminescence Detection application brief that demonstrates cell viability technologies that use absorbance, fluorescence, or luminescence detection >

Advantages of Multimode Readers

Flexibility and efficiency

By consolidating multiple detection modes into a single platform, multimode readers enhance workflow efficiency. Laboratories can seamlessly switch between applications without reconfiguring equipment, saving time and reducing errors.

Cost-effectiveness

Investing in a single, multi-functional instrument reduces overall costs compared to purchasing and maintaining separate devices for different assays. Additionally, modern instruments come with extended lifespans and robust support services, ensuring long-term value.

Ease of use

Modern systems, like the Agilent BioTek Synergy Multimode Readers offered by Chemetrix, feature intuitive software interfaces, automated calibration, and built-in protocols. These innovations minimise the learning curve, making the instruments accessible to new and experienced users alike.

 

Trends in Multimode Microplate Reader development

The development of multimode readers continues to evolve in response to laboratory demands:

  • Miniaturisation and Automation: Instruments are becoming more compact and are integrating seamlessly into automated workflows, reducing hands-on time and increasing throughput.
  • Enhanced Sensitivity: Advances in optical systems and detection technologies enable the identification of low-abundance analytes with unprecedented accuracy.
  • Cloud-Based Data Management: Modern readers increasingly offer cloud integration, facilitating remote monitoring and data sharing across teams.

 

Trust Chemetrix as your partner in advanced laboratory solutions

Chemetrix provides cutting-edge solutions tailored to the needs of modern laboratories. With applications spanning life sciences, environmental testing, and pharmaceutical development, these systems are designed to deliver precise, reproducible results across a broad spectrum of research fields.

By offering industry-leading instruments such as Agilent BioTek Synergy Multimode Readers, Chemetrix empowers researchers with the tools to meet complex challenges. The Agilent BioTek Synergy H1 multimode reader is a configurable multimode microplate reader, with monochromator-based optics for flexibility, filter-based optics for sensitivity, or both. This hybrid technology offers application versatility and excellent performance in a modular platform to expand as your laboratory’s needs change. Its upgradeable, modular design allows for added detection modes, gas control, and dual reagent injectors as your laboratory workflows evolve.

When you need to bring cost-effective, automated spinning-disk confocal microscopy to a lab, the Agilent BioTek Cytation 10 is a bench-size microplate imaging and analysis workhorse. It combines automated confocal and widefield microscopy with conventional multimode microplate reading in a unique, proprietary design. The automated water-immersion objectives capture more light, driving lower exposure times and reducing phototoxic impacts on live cells. It’s an easy-to-use instrument that delivers high quality analysis.

📚 Watch the Moving Cell Migration Research Forward with BioTek webinar to learn how BioTek’s innovative instruments can simplify your workflows and deliver reliable, reproducible results >

Agilent BioTek Cytation C10 Confocal Imaging Reader
Agilent BioTek Synergy H1 Multimode Reader

By partnering with us, laboratories gain access not only to advanced technology but also to comprehensive support services, including training, maintenance, and technical assistance. This ensures that researchers can focus on innovation while relying on robust, reliable equipment.

📚 Download the 3D Cell Culture: A Review of Current Techniques white paper and learn how 3D systems are advancing research in drug development, disease modelling, and tissue engineering >

The versatility and precision of multi-mode microplate readers have made them essential in modern laboratories. From nucleic acid quantification to enzyme kinetics, these instruments offer unparalleled capabilities that streamline workflows and drive innovation. By delivering on our commitment to top-tier laboratory solutions, researchers can confidently tackle emerging challenges and push the boundaries of discovery.

]]> https://chemetrix.co.za/the-role-of-multi-mode-microplate-readers-in-modern-laboratories/feed/ 0 High-Throughput Intact Native Protein Analysis https://chemetrix.co.za/high-throughput-intact-native-protein-analysis/ https://chemetrix.co.za/high-throughput-intact-native-protein-analysis/#respond Tue, 06 Sep 2022 14:43:18 +0000 https://chemetrix.co.za/?p=4699 Continue reading "High-Throughput Intact Native Protein Analysis"]]> Ion mobility-mass spectrometry has become a valuable analytical tool in native protein analysis. In protein structure studies, ion mobility spectrometry provides rotationally averaged collision cross-section values that correlate to size and shape of the biomolecule. For proteins, ground state CCS and accurate mass are not adequate to identify different proteins. Therefore, the introduction of gas phase unfolding followed by ion mobility measurements provide unique fingerprints for native protein analysis. This collision-induced unfolding (CIU) technique can be utilized to identify proteins and protein complexes. Typical CIU experiments utilize static nano-ESI or standard ESI using a syringe pump for sample introduction which is difficult to automate. In this study, we have developed a new automated sample introduction method for high-throughput CIU experiments which can be adapted for IgG and other proteins.

 

Speaker

Sheher Banu Mohsin, Ph.D.
Senior Applications Scientist
Agilent Technologies, Inc.

 

Sheher Mohsin is a senior applications scientist at Agilent Technologies. She received her Ph. D in physical chemistry from the University of Illinois and an MBA from Rockhurst University. She started her career at the US Environmental Protection Agency working on dioxin analysis with high-resolution mass spectrometers. She later joined Bayer and worked in the special analysis lab using mass spectrometry to solve problems in synthesis, impurity determination and submission of final product impurity profile to regulatory agencies. Sheher’s current focus is on lipidomics using GC, LC and SFC separations and mass spectrometry. Sheher collaborates with academic and government researchers working on complex problems to come up with innovative, simplified workflows using the latest tools in separation and mass spectrometry.

 

Register and watch on demand >

 

]]> https://chemetrix.co.za/high-throughput-intact-native-protein-analysis/feed/ 0